Quality of work and employment, industrial relations and restructuring in Turkey.

Despite the rapid economic growth of recent years, Turkey has not been successful in translating this growth into the creation of sufficient numbers of new jobs. Employment grew by only 1.2% in 2005 and by 1.3% in 2006, increasing slightly to just 1.8% in the third quarter of 2007. The country’s unemployment rate has remained at about 10%– 11% for each year since the start of the economic recovery in 2002. Unemployment is particularly high among young people aged 15–24 years, reaching 19% in recent years

What happens when oral tuberculosis is not treated?

Pulmonary tuberculosis (TB) is the most important form of the disease, although infection may also occur by way of the intestinal tract, tonsils and skin. Oral lesions consist of persistent ulcers or granulomatous masses. A 50 year old man had been diagnosed “necrotising granulomatous inflammation” following a biopsy of a lesion on lower lip, 21 months before at a medical centre. A chest-X-ray had not been performed and he had not been given any advise in respect of treatment. He was admitted to the hospital with cough, sputum, weakness, weight loss and lesions on his lower lip. In radiology, it was detected that he had supraclavicular, submental, cervical, mediastinal lymphadenopathies, pulmonary infiltrations with cavities, thickening and roughness on left oropharengial tonsil, thickenning on inner parts of larynx and bilateral surrenal thickening. The biopsy of lesions on larynx, tonsil and epiglottis revealed “necrotising granulomatous inflammation” and histopathology supported TB infection. Sputum acid-fast bacilli was positive and culture was positive for Mycobacterium tuberculosis complex. Two months of combination treatment resulted in a gradual relief of the symptoms, radiological response, disappearing of neck swelling and healing of lesions on lip, tonsil and larynx. Although unusual oral cavity manifestations of TB are rare, clinicians should be aware of possible occurrance

3-D sediment-basement tomography of the Northern Marmara trough by a dense OBS network at the nodes of a grid of controlled source profiles along the North Anatolian fault

A 3-D tomographic inversion of first arrival times of shot profiles recorded by a dense 2-D OBS network provides an unprecedented constraint on the P-wave velocities heterogeneity of the upper-crustal part of the North Marmara Trough (NMT), over a region of 180 km long by 50 km wide. One of the specific aims of this controlled source tomography is to provide a 3-D initial model for the local earthquake tomography (LET). Hence, in an original way, the controlled source inversion has been performed by using a code dedicated to LET. After several tests to check the results trade-off with the inversion parameters, we build up a 3-D a priori velocity model, in which the sea-bottom topography, the acoustic and the crystalline basements and the Moho interfaces have been considered. The reliability of the obtained features has been checked by checkerboard tests and also by their comparison with the deep-penetration multichannel seismic profiles, and with the wide-angle reflection and refraction modelled profiles. This study provides the first 3-D view of the basement topography along the active North Anatolian fault beneath the Marmara Sea, even beneath the deepest part of three sedimentary basins of NMT. Clear basement depressions reaching down 6 km depth below the sea level (bsl) have been found beneath these basins. The North Imralı Basin located on the southern continental shelf is observed with a similar sedimentary thickness as its northern neighbours. Between Central and Çınarcık basins, the Central High rises up to 3 km depth below (bsl). Its crest position is offset by 10 km northwestward relatively to the bathymetric crest. On the contrary, Tekirdağ and Central basins appear linked, forming a 60-km-long basement depression. Beneath the bathymetric relief of Western High low velocities are observed down to 6 km depth (bsl) and no basement high have been found. The obtained 3-D Vp heterogeneity model allows the consideration of the 3-D supracrustal heterogeneity into the future earthquake relocations in this region. The topographic map of the pre-kinematic basement offers the possibility to take into account the locking depth variations in future geohazard estimations by geomechanical modelling in this region

Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: A case study with the metastatic breast cancer cell line MDA-MB-231

Metabolome characterisation is a powerful tool in oncology. To obtain a valid description of the intracellular metabolome, two of the preparatory steps are crucial, namely washing and quenching. Washing must effectively remove the extracellular media components and quenching should stop the metabolic activities within the cell, without altering the membrane integrity of the cell. Therefore, it is important to evaluate the efficiency of the washing and quenching solvents. In this study, we employed two previously optimised protocols for simultaneous quenching and extraction, and investigated the effects of a number of washing steps/solvents and quenching solvent additives, on metabolite leakage from the adherent metastatic breast cancer cell line MDA-MB-231. We explored five washing protocols and five quenching protocols (including a control for each), and assessed for effectiveness by detecting ATP in the medium and cell morphology changes through scanning electron microscopy (SEM) analyses. Furthermore, we studied the overall recovery of eleven different metabolite classes using the GC-MS technique and compared the results with those obtained from the ATP assay and SEM analysis. Our data demonstrate that a single washing step with PBS and quenching with 60% methanol supplemented with 70 mM HEPES (−50 °C) results in minimum leakage of intracellular metabolites. Little or no interference of PBS (used in washing) and methanol/HEPES (used in quenching) on the subsequent GC-MS analysis step was noted. Together, these findings provide for the first time a systematic study into the washing and quenching steps of the metabolomics workflow for studying adherent mammalian cells, which we believe will improve reliability in the application of metabolomics technology to study adherent mammalian cell metabolism

Observation of the spread of slow deformation in Greece following the breakup of the slab

International audienc

Authigenic carbonates from the Cascadia subduction zone and their relation to gas hydrate stability

Authigenic carbonates are intercalated with massive gas hydrates in sediments of the Cascadia margin. The deposits were recovered from the uppermost 50 cm of sediments on the southern summit of the Hydrate Ridge during the RV Sonne cruise SO110. Two carbonate lithologies that differ in chemistry, mineralogy, and fabric make up these deposits. Microcrystalline high-magnesium calcite (14 to 19 mol% MgCO3) and aragonite are present in both semiconsolidated sediments and carbonate-cemented clasts. Aragonite occurs also as a pure phase without sediment impurities. It is formed by precipitation in cavities as botryoidal and isopachous aggregates within pure white, massive gas hydrate. Variations in oxygen isotope values of the carbonates reflect the mineralogical composition and define two end members: a Mg-calcite with δ18O =4.86‰ PDB and an aragonite with δ18O =3.68‰ PDB. On the basis of the ambient bottom-water temperature and accepted equations for oxygen isotope fractionation, we show that the aragonite phase formed in equilibrium with its pore-water environment, and that the Mg-calcite appears to have precipitated from pore fluids enriched in 18O. Oxygen isotope enrichment probably originates from hydrate water released during gas-hydrate destabilization

Comparison between transfer path analysis methods on an electric vehicle

A comparison between transfer path analysis and operational path analysis methods using an electric vehicle is presented in this study. Structure-borne noise paths to the cabin from different engine and suspension points have been considered. To realise these methods, two types of test have been performed; operational tests on a rolling road and hammer tests in static conditions. The main aim of this work is assessing the critical paths which are transmitting the structure-borne vibrations from the electric vehicle?s vibration sources to the driver?s ear. This assessment includes the analysis of the noise contribution of each path depending on the frequency and vehicle speed range and moreover, the assessment of the path noise impact for harmonic orders which arise due to the physical components of the electric vehicle. Furthermore, the applicability of these methods to electric vehicles is assessed as these techniques have been extensively used for vehicles powered with internal combustion engines.The authors would like to acknowledge the COST ACTION TU1105 for supporting this research

Reprogramming of Escherichia coli K-12 Metabolism during the Initial Phase of Transition from an Anaerobic to a Micro-Aerobic Environment

Background: Many bacteria undergo transitions between environments with differing O2 availabilities as part of their natural lifestyles and during biotechnological processes. However, the dynamics of adaptation when bacteria experience changes in O2 availability are understudied. The model bacterium and facultative anaerobe Escherichia coli K-12 provides an ideal system for exploring this process. Methods and Findings: Time-resolved transcript profiles of E. coli K-12 during the initial phase of transition from anaerobic to micro-aerobic conditions revealed a reprogramming of gene expression consistent with a switch from fermentative to respiratory metabolism. The changes in transcript abundance were matched by changes in the abundances of selected central metabolic proteins. A probabilistic state space model was used to infer the activities of two key regulators, FNR (O2 sensing) and PdhR (pyruvate sensing). The model implied that both regulators were rapidly inactivated during the transition from an anaerobic to a micro-aerobic environment. Analysis of the external metabolome and protein levels suggested that the cultures transit through different physiological states during the process of adaptation, characterized by the rapid inactivation of pyruvate formate-lyase (PFL), a slower induction of pyruvate dehydrogenase complex (PDHC) activity and transient excretion of pyruvate, consistent with the predicted inactivation of PdhR and FNR. Conclusion: Perturbation of anaerobic steady-state cultures by introduction of a limited supply of O2 combined with time-resolved transcript, protein and metabolite profiling, and probabilistic modeling has revealed that pyruvate (sensed by PdhR) is a key metabolic signal in coordinating the reprogramming of E. coli K-12 gene expression by working alongside the O2 sensor FNR during transition from anaerobic to micro-aerobic conditions

Металлизация поверхности керамики В4С комбинированным электронно-ионно-плазменным методом

Объектом исследования является керамика В4С с металлизированной поверхностью. Цель работы – исследование структуры и свойств систем «пленка (Ti)/(В4С-керамика) подложка» и «пленка (Cu)/(В4С-керамика) подложка», обработанных интенсивным импульсным электронным пучком. В процессе исследования проводились рентгенофазовые исследование; исследование поверхности и излома образцов методом сканирующей электронной микроскопии; исследование микротвердости и коэффициента вязкости разрушения образов. В результате исследования установлено формированию в поверхностном слое толщиной (5-7) мкм многофазной структуры эвтектического типа, состоящей из кристаллитов карбида бора, диборида титана и титана; снижение микротвердости модифицированных образцов керамики Ti/В4С в ≈1,2 раза относительно микротвёрдости исходных образцов. Основные конструктивные, технологические и технико-эксплуатационные характеристики: температура разложения >2450 °C; плотность 2,52 г/см³; теплопроводность 121 Вт/(мК) при 300 °К; микротвёрдость 49,1 ГПа; модуль упругости 450 ГПа; полупроводник p-типа.The object of the research is B4C ceramics with metallized surface. Objective - to study the structure and properties of systems "film (Ti) / (B4C-ceramic) substrate" and "film (Cu) / (B4C-ceramic) substrate" treated with intense pulsed electron beam. The study carried out X-ray phase study; study the surface and break the samples by scanning electron microscopy; study of microhardness and fracture toughness ratio images. The study showed the formation of a surface layer (5-7) m multiphase eutectic structure consisting of crystallites of boron carbide, titanium diboride, and titanium; decrease in microhardness modified ceramic samples Ti / B4C in ≈1,2 times the microhardness of the initial samples. The basic constructive, technological and technical and operational characteristics: the decomposition temperature of> 2450 ° C; density 2.52 g / cm³; thermal conductivity of 121 W / (mK) at 300 ° K; microhardness 49.1 GPa; modulus 450 GPa; p-type semiconductor